The GENOMEMASKER package is able to mask the entire human genome for non-unique primers within 6 hours and find locations of all binding sites for 10 000 designed primer pairs within 10 minutes.
GenomeMasker 1.3 / GenomeTester 1.3 / SNPmasker 1.1 Posted on 7 7 Categories PCR / Primer Design Tags AAScan, High-throughput Scanning, Mutagenesis, MutantChecker, PCR Cloning, Primer Design, Sequence Analysis Oligospawn – Oligo Design from large-scale cDNA Sequences Sun D1, Ostermaier MK, Heydenreich FM, Mayer D, Jaussi R, Standfuss J, Veprintsev DB. eCollection 2013.ĪAscan, PCRdesign and MutantChecker: a suite of programs for primer design and sequence analysis for high-throughput scanning mutagenesis. Laboratory of Biomolecular Research (LBR) PCR Cloning – design of primers for ligation-independent cloning MutantChecker – helps to analyse the sequenceing results from scanning mutagenesis
#AMPLIFX CENA SOFTWARE#
:: MORE INFORMATION Posted on 8 8 Categories PCR / Primer Design Tags AmplifX, Design, Manage, PCR, Primer Leave a comment on AmplifX 2.1.1 – Manage & Design Primers for PCR AAScan 2.2 / MutantChecker 1.3 / PCR Cloning 1.5 – Primer Design and Sequence Analysis for High-throughput Scanning MutagenesisĪAScan 2.2 / MutantChecker 1.3 / PCR Cloning 1.5ĪAscan, PCRdesign and MutantChecker is a suite of programs for primer design and sequence analysis for high-throughput scanning mutagenesis.ĪAScan (formely AlaScan) – batch primer design software for scanning mutagenesis Some information is associated with each primer some automaticelly computed by AmplifX (like TM, Quality, length) and others given by the user (name, comments,…) This allows general aspects of primer management (sequences and real tubes). Neurosci Lett 2003 Mar 13 339(1): 62-66 Posted on 9 9 Categories PCR / Primer Design Tags Analysis, Data, LinRegPCR, Quantitative PCR Leave a comment on LinRegPCR 20210614 – Analysis of Quantitative PCR Data AlleleID 7.85 – Assay Design for Bacterial IdentificationĪmplifX is a software to search through a collection of primers, such as any molecular biologist has in his refrigerators, to find those which can be use to amplify a fragment into a target sequence, for example, and particularly, to design strategies to screen recombinant clones by PCR. (2003)Īssumption-free analysis of quantitative real-time PCR data Ramakers C, Ruijter JM, Deprez RH, Moorman AF. Ilgun Heart Failure Research Center (HFRC) With the mean PCR efficiency per amplicon, the Ct value per sample and the fluorescence threshold set to determnine the Ct, the starting concentration per sample, expressed in arbitrary fluorescence units, is calculated Then a Window-of-Linearity is set and PCR efficiencies per sample are calculated. The program determines a baseline fluorescence and does a baseline subtraction. LinRegPCR is a program for the analysis of quantitative RT-PCR (qPCR) data resulting from monitoring the PCR reaction with SYBR green or similar fluorescent dyes.
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